Abstract
The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons containedinside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tke, at least one promoter for expression of the structural genes in thebacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transformed, is also provided. The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditionalfermentation technology, because they are stable for expression in a non-selection medium.
| Original language | American English |
|---|---|
| Patent number | 7,354,755 B2 |
| State | Published - 2008 |
NREL Publication Number
- NREL/PT-510-43382