Strain, Biochemistry, and Cultivation-Dependent Measurement Variability of Algal Biomass Composition

Lieve Laurens, Stefanie Van Wychen, Philip Pienkos, Jordan McAllister, Sarah Arrowsmith, Thomas Dempster, John McGowen

Research output: Contribution to journalArticlepeer-review

78 Scopus Citations

Abstract

Accurate compositional analysis in biofuel feedstocks is imperative; the yields of individual components can define the economics of an entire process. In the nascent industry of algal biofuels and bioproducts, analytical methods that have been deemed acceptable for decades are suddenly critical for commercialization. We tackled the question of how the strain and biochemical makeup of algal cells affect chemical measurements. We selected a set of six procedures (two each for lipids, protein, and carbohydrates): three rapid fingerprinting methods and three advanced chromatography-based methods. All methods were used to measure the composition of 100 samples from three strains: Scenedesmus sp., Chlorella sp., and Nannochloropsis sp. The data presented point not only to species-specific discrepancies but also to cell biochemistry-related discrepancies. There are cases where two respective methods agree but the differences are often significant with over- or underestimation of up to 90%, likely due to chemical interferences with the rapid spectrophotometric measurements. We provide background on the chemistry of interfering reactions for the fingerprinting methods and conclude that for accurate compositional analysis of algae and process and mass balance closure, emphasis should be placed on unambiguous characterization using methods where individual components are measured independently.

Original languageAmerican English
Pages (from-to)86-95
Number of pages10
JournalAnalytical Biochemistry
Volume452
Issue number1
DOIs
StatePublished - 2014

NREL Publication Number

  • NREL/JA-5100-60224

Keywords

  • Analytical methods
  • Biochemical composition
  • Carbohydrates
  • Lipids
  • Microalgae
  • Proteins

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