Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction

Seon Ah Kim, Gregg Beckham, Mikael Gudmundsson, Miao Wu, Takuya Ishida, Majid Momeni, Gustav Vaaje-Kolstad, Daniel Lundberg, Antoine Royant, Jerry Ståhlberg, Vincent Eijsink, Mats Sandgren

Research output: Contribution to journalArticlepeer-review

96 Scopus Citations

Abstract

Background: Lytic polysaccharide monooxygenases (LPMOs) exhibit a copper center that binds dioxygen for catalysis. Results: We present LPMO structures from Cu(II) to Cu(I) and analyze the transition with quantum mechanical calculations. Conclusion: Reduction changes the copper coordination state but requires only minor structural and electronic changes. Significance: These structures provide insight into LPMO catalytic activation for further mechanistic studies.

Original languageAmerican English
Pages (from-to)18782-18792
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number27
DOIs
StatePublished - 4 Jul 2014

NREL Publication Number

  • NREL/JA-5100-62549

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