Surface-Enhanced Raman Scattering Spectroscopy of Photosynthetic Membranes and Complexes

Michael Seibert, Rafael Picorel, Jae Ho Kim, Therese M. Cotton

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

18 Scopus Citations


Surface-enhanced Raman scattering (SERS) results when ion complexes, molecules, or chromophores adsorbed onto or near roughened silver, gold, or copper substrates are excited with laser light. Surface enhanced resonance Raman scattering (SERRS) occurs when the laser excitation wavelength is in resonance with an electronic transition in the adsorbate and provides additional enhancement. The major advantages of SERS/SERRS include extreme analytical sensitivity, molecular selectivity, minimization of fluorescence, and distance sensitivity. SERRS has considerable potential for analytical applications and for fundamental studies of biomolecular structure on surfaces. Membrane studies suggest that SERRS can provide qualitative information about the location and orientation of chromophores within the membrane. Future developments should include the use of new detectors, which have very low dark counts and can be used to integrate signals for long time periods. It should be possible to obtain unenhanced Raman spectra on any surface using this approach. Also, the use of new lasers, such as the titanium: sapphire laser allows excitation throughout the red region of the electromagnetic spectrum (650–1000 nm). This is valuable for studies of biological samples on gold substrates.

Original languageAmerican English
Title of host publicationMethods in Enzymology
EditorsL. Packer
Number of pages12
StatePublished - 1 Jan 1992
Externally publishedYes

Publication series

NameMethods in Enzymology
PublisherAcademic Press Inc.
ISSN (Print)0076-6879

NREL Publication Number

  • ACNR/CH-15949


Dive into the research topics of 'Surface-Enhanced Raman Scattering Spectroscopy of Photosynthetic Membranes and Complexes'. Together they form a unique fingerprint.

Cite this