TY - JOUR
T1 - Survey of the Anaerobic Metabolism of Various Laboratory Wild-Type Chlamydomonas reinhardtii Strains
AU - Ghirardi, Maria
AU - Subramanian, Venkataramanan
AU - Smolinski, Sharon
AU - Xiong, Wei
AU - Wecker, Matt
AU - Antonio, Regina
AU - Gonzalez-Ballester, David
AU - Dubini, Alexandra
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018
Y1 - 2018
N2 - Given the wide diversity of wild-type Chlamydomonas reinhardtii genotypes due to the presence of genes belonging to either of different gene haplotypes (Gallaher, S.D., Fitz-Gibon, S.R., Glaesener, A.G., Pellegrini, M. and Merchant, S.S. 2015, The Plant Cell, doi 10.1105/tpc.15.00508), we performed a phenotypic characterization of representative strains whose anaerobic metabolism has been studied either by our laboratory or by others. Our results reveal major differences in the levels of H2 production and in the pattern of fermentative metabolite generation, highlighting the activation of different fermentative pathways in each organism. Some of the differences could be attributed to differences in the haplotypes of the various genes involved in these pathways. Moreover, the nature of the metabolites produced under dark anaerobiosis versus sulfur-deprivation-induced anaerobiosis are different in the analyzed strains, emphasizing the differential activation of metabolic pathways promoted under the two conditions and highlighting the importance of utilizing appropriate C. reinhardtii strains for addressing specific research questions.
AB - Given the wide diversity of wild-type Chlamydomonas reinhardtii genotypes due to the presence of genes belonging to either of different gene haplotypes (Gallaher, S.D., Fitz-Gibon, S.R., Glaesener, A.G., Pellegrini, M. and Merchant, S.S. 2015, The Plant Cell, doi 10.1105/tpc.15.00508), we performed a phenotypic characterization of representative strains whose anaerobic metabolism has been studied either by our laboratory or by others. Our results reveal major differences in the levels of H2 production and in the pattern of fermentative metabolite generation, highlighting the activation of different fermentative pathways in each organism. Some of the differences could be attributed to differences in the haplotypes of the various genes involved in these pathways. Moreover, the nature of the metabolites produced under dark anaerobiosis versus sulfur-deprivation-induced anaerobiosis are different in the analyzed strains, emphasizing the differential activation of metabolic pathways promoted under the two conditions and highlighting the importance of utilizing appropriate C. reinhardtii strains for addressing specific research questions.
KW - Anaerobiosis
KW - Chlamydomonas reinhardtii
KW - Metabolites
KW - Sulfur deprivation
KW - Wild type
UR - http://www.scopus.com/inward/record.url?scp=85053377956&partnerID=8YFLogxK
U2 - 10.1016/j.algal.2018.05.002
DO - 10.1016/j.algal.2018.05.002
M3 - Article
AN - SCOPUS:85053377956
SN - 2211-9264
VL - 35
SP - 355
EP - 361
JO - Algal Research
JF - Algal Research
ER -