Survey of the Anaerobic Metabolism of Various Laboratory Wild-Type Chlamydomonas reinhardtii Strains

Maria Ghirardi, Venkataramanan Subramanian, Sharon Smolinski, Wei Xiong, Matt Wecker, Regina Antonio, David Gonzalez-Ballester, Alexandra Dubini

Research output: Contribution to journalArticlepeer-review

6 Scopus Citations


Given the wide diversity of wild-type Chlamydomonas reinhardtii genotypes due to the presence of genes belonging to either of different gene haplotypes (Gallaher, S.D., Fitz-Gibon, S.R., Glaesener, A.G., Pellegrini, M. and Merchant, S.S. 2015, The Plant Cell, doi 10.1105/tpc.15.00508), we performed a phenotypic characterization of representative strains whose anaerobic metabolism has been studied either by our laboratory or by others. Our results reveal major differences in the levels of H2 production and in the pattern of fermentative metabolite generation, highlighting the activation of different fermentative pathways in each organism. Some of the differences could be attributed to differences in the haplotypes of the various genes involved in these pathways. Moreover, the nature of the metabolites produced under dark anaerobiosis versus sulfur-deprivation-induced anaerobiosis are different in the analyzed strains, emphasizing the differential activation of metabolic pathways promoted under the two conditions and highlighting the importance of utilizing appropriate C. reinhardtii strains for addressing specific research questions.

Original languageAmerican English
Pages (from-to)355-361
Number of pages7
JournalAlgal Research
StatePublished - 2018

Bibliographical note

Publisher Copyright:
© 2018 Elsevier B.V.

NREL Publication Number

  • NREL/JA-2A00-71082


  • Anaerobiosis
  • Chlamydomonas reinhardtii
  • Metabolites
  • Sulfur deprivation
  • Wild type


Dive into the research topics of 'Survey of the Anaerobic Metabolism of Various Laboratory Wild-Type Chlamydomonas reinhardtii Strains'. Together they form a unique fingerprint.

Cite this