Sweet Sorghum Bagasse and Corn Stover Serving as Substrates for Producing Sophorolipids

Xiaowen Chen, Abdul Samad, Ji Zhang, Da Chen, Yanna Liang, Melvin Tucker

Research output: Contribution to journalArticlepeer-review

32 Scopus Citations


To make the process of producing sophorolipids by Candida bombicola truly sustainable, we investigated production of these biosurfactants on biomass hydrolysates. This study revealed: (1) yield of sophorolipds on bagasse hydrolysate decreased from 0.56 to 0.54 and to 0.37 g/g carbon source when yellow grease was dosed at 10, 40 and 60 g/L, respectively. In the same order, concentration of sophorolipids was 35.9, 41.9, and 39.3 g/L; (2) under similar conditions, sophorolipid yield was 0.12, 0.05 and 0.04 g/g carbon source when corn stover hydrolysate was mixed with soybean oil at 10, 20 and 40 g/L. Sophorolipid concentration was 11.6, 4.9, and 3.9 g/L for the three oil doses from low to high; and (3) when corn stover hydrolysate and yellow grease served as the substrates for cultivating the yeast in a fermentor, sophorolipid concentration reached 52.1 g/L. Upon further optimization, sophorolipids production from ligocellulose will be indeed sustainable.

Original languageAmerican English
Pages (from-to)353-362
Number of pages10
JournalJournal of Industrial Microbiology and Biotechnology
Issue number3
StatePublished - 1 Mar 2017

Bibliographical note

Publisher Copyright:
© 2016, Society for Industrial Microbiology and Biotechnology.

NREL Publication Number

  • NREL/JA-5100-67752


  • Candida (Starmerella) bombicola
  • Corn stover
  • Deacetylated and disc refined (DDR)
  • Sophorolipids
  • Sweet sorghum bagasse


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