Tandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids

Karin A.Zemski Berry; Robert M. Barkley; Joseph J. Berry; Joseph A. Hankin; Emmy Hoyes; Jeffery M. Brown; Robert C. Murphy

doi:10.1021/acs.analchem.6b04047

Tandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids

Karin A.Zemski Berry, Robert M. Barkley, Joseph J. Berry, Joseph A. Hankin, Emmy Hoyes, Jeffery M. Brown, Robert C. Murphy

Materials, Chemical, and Computational Science

Research output: Contribution to journal › Article › peer-review

26 Scopus Citations

Abstract

Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. It was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.

Original language	American English
Pages (from-to)	916-921
Number of pages	6
Journal	Analytical Chemistry
Volume	89
Issue number	1
DOIs	https://doi.org/10.1021/acs.analchem.6b04047 https://doi.org/10.1021/acs.analchem.6b04047
State	Published - 3 Jan 2017

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

NREL Publication Number

NREL/JA-5K00-67567

Keywords

biology
mass spectrometry
phospholipids

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abstract = "Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. It was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.",

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AU - Berry, Karin A.Zemski

AU - Barkley, Robert M.

AU - Berry, Joseph J.

AU - Hankin, Joseph A.

AU - Hoyes, Emmy

AU - Brown, Jeffery M.

AU - Murphy, Robert C.

PY - 2017/1/3

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AB - Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. It was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.

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Tandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids

Abstract

Bibliographical note

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Keywords

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