Survey and Analysis of Commercial Cellulase Preparations Suitable for Biomass Conversion to Ethanol

R. A. Nieves; C. I. Ehrman; W. S. Adney; R. T. Elander; M. E. Himmel

doi:10.1023/A:1008871205580

Survey and Analysis of Commercial Cellulase Preparations Suitable for Biomass Conversion to Ethanol

R. A. Nieves, C. I. Ehrman, W. S. Adney, R. T. Elander, M. E. Himmel

Bioenergy Science and Technology

National Renewable Energy Laboratory

Research output: Contribution to journal › Article › peer-review

128 Scopus Citations

Abstract

Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-β-D-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger β-D-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger.

Original language	American English
Pages (from-to)	301-304
Number of pages	4
Journal	World Journal of Microbiology and Biotechnology
Volume	14
Issue number	2
DOIs	https://doi.org/10.1023/A:1008871205580 https://doi.org/10.1023/A:1008871205580
State	Published - Mar 1997

NREL Publication Number

NREL/JA-580-22260

Keywords

Cellobiase
Cellulase
Industrial enzyme preparations
β-glucosidase

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abstract = "Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-β-D-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger β-D-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger.",

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AU - Nieves, R. A.

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AU - Adney, W. S.

AU - Elander, R. T.

AU - Himmel, M. E.

PY - 1997/3

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AB - Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-β-D-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger β-D-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger.

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Survey and Analysis of Commercial Cellulase Preparations Suitable for Biomass Conversion to Ethanol

Abstract

NREL Publication Number

Keywords

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