Technical Communication: Survey and Analysis of Commercial Cellulase Preparations Suitable for Biomass Conversion to Ethanol

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples weresubjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-beta-d-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger Beta-D-glucosidase were developed. Eleven of the cellulases tested were of T.reesei or T. viride origin and two were from A. niger.
    Original languageAmerican English
    Pages (from-to)301-304
    Number of pages4
    JournalWorld Journal of Microbiology and Biotechnology
    Volume14
    Issue number2
    DOIs
    StatePublished - 1998

    NREL Publication Number

    • NREL/JA-580-22260

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