The Multi Domain Caldicellulosiruptor bescii CelA Cellulase Excels at the Hydrolysis of Crystalline Cellulose: Article No. 9622

Yannick Bomble, Roman Brunecky, Bryon Donohoe, John Yarbrough, Ashutosh Mittal, Daehwan Chung, Michael Himmel, Brian Scott, Hanshu Ding, Larry Taylor III, Jordan Russell, Janet Westpheling, Sarah Teter

Research output: Contribution to journalArticlepeer-review

38 Scopus Citations

Abstract

The crystalline nature of cellulose microfibrils is one of the key factors influencing biomass recalcitrance which is a key technical and economic barrier to overcome to make cellulosic biofuels a commercial reality. To date, all known fungal enzymes tested have great difficulty degrading highly crystalline cellulosic substrates. We have demonstrated that the CelA cellulase from Caldicellulosiruptor bescii degrades highly crystalline cellulose as well as low crystallinity substrates making it the only known cellulase to function well on highly crystalline cellulose. Unlike the secretomes of cellulolytic fungi, which typically comprise multiple, single catalytic domain enzymes for biomass degradation, some bacterial systems employ an alternative strategy that utilizes multi-catalytic domain cellulases. Additionally, CelA is extremely thermostable and highly active at elevated temperatures, unlike commercial fungal cellulases. Furthermore we have determined that the factors negatively affecting digestion of lignocellulosic materials by C. bescii enzyme cocktails containing CelA appear to be significantly different from the performance barriers affecting fungal cellulases. Here, we explore the activity and degradation mechanism of CelA on a variety of pretreated substrates to better understand how the different bulk components of biomass, such as xylan and lignin, impact its performance.
Original languageAmerican English
Number of pages17
JournalScientific Reports
Volume7
DOIs
StatePublished - 2017

NREL Publication Number

  • NREL/JA-2700-68492

Keywords

  • biomass
  • cellulosic biofuels
  • recalcitrance

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