Thermal Stabilization of Fungal Beta-Glucosidase Through Glutaraldehyde Crosslinking

J. O. Baker, K. K. Oh, K. Grohmann, M. E. Himmel

Research output: Contribution to journalArticlepeer-review

23 Scopus Citations


The thermal stability of a purified Aspergillus niger β-glucosidase (EC has been dramatically increased by intramolecular crosslinking with glutaraldehyde at pH 9.0, followed by borohydride reduction. The stabilization has been shown to be due to the formation of a distinct subpopulation of enzyme molecules, the activity of which has a half-life at 65°C of approximately 40 hours, which is some 80 times as long as that of the native enzyme at this temperature.

Original languageAmerican English
Pages (from-to)325-330
Number of pages6
JournalBiotechnology Letters
Issue number5
StatePublished - May 1988

NREL Publication Number

  • ACNR/JA-232-10214


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