Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

9 Scopus Citations

Abstract

Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.

Original languageAmerican English
Title of host publicationBiofuels from Algae: Methods and Protocols
Subtitle of host publicationMethods in Molecular Biology
EditorsK. Spilling
PublisherHumana Press Inc.
Pages233-242
Number of pages10
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume1980
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media New York.

NREL Publication Number

  • NREL/CH-5100-71450

Keywords

  • Amino acids
  • Nitrogen analysis
  • Nitrogen-to-protein conversion factor
  • Protein

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