Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

Lieve Laurens; Jessica Olstad-Thompson; David Templeton

doi:10.1007/7651_2018_126

Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

Lieve Laurens, Jessica Olstad-Thompson, David Templeton

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

12 Scopus Citations

Abstract

Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.

Original language	American English
Title of host publication	Biofuels from Algae: Methods and Protocols
Subtitle of host publication	Methods in Molecular Biology
Editors	K. Spilling
Publisher	Humana Press Inc.
Pages	233-242
Number of pages	10
DOIs	https://doi.org/10.1007/7651_2018_126 https://doi.org/10.1007/7651_2018_126
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	1980
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media New York.

NREL Publication Number

NREL/CH-5100-71450

Keywords

Amino acids
Nitrogen analysis
Nitrogen-to-protein conversion factor
Protein

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Laurens, L., Olstad-Thompson, J., & Templeton, D. (2020). Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. In K. Spilling (Ed.), Biofuels from Algae: Methods and Protocols: Methods in Molecular Biology (pp. 233-242). (Methods in Molecular Biology; Vol. 1980). Humana Press Inc.. https://doi.org/10.1007/7651_2018_126, https://doi.org/10.1007/7651_2018_126

Laurens, Lieve ; Olstad-Thompson, Jessica ; Templeton, David. / Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. Biofuels from Algae: Methods and Protocols: Methods in Molecular Biology. editor / K. Spilling. Humana Press Inc., 2020. pp. 233-242 (Methods in Molecular Biology).

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abstract = "Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.",

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doi = "10.1007/7651_2018_126",

language = "American English",

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Laurens, L , Olstad-Thompson, J & Templeton, D 2020, Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. in K Spilling (ed.), Biofuels from Algae: Methods and Protocols: Methods in Molecular Biology. Methods in Molecular Biology, vol. 1980, Humana Press Inc., pp. 233-242. https://doi.org/10.1007/7651_2018_126, https://doi.org/10.1007/7651_2018_126

Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. / Laurens, Lieve ; Olstad-Thompson, Jessica; Templeton, David.
Biofuels from Algae: Methods and Protocols: Methods in Molecular Biology. ed. / K. Spilling. Humana Press Inc., 2020. p. 233-242 (Methods in Molecular Biology; Vol. 1980).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

AU - Laurens, Lieve

AU - Olstad-Thompson, Jessica

AU - Templeton, David

PY - 2020

Y1 - 2020

N2 - Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.

AB - Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.

KW - Amino acids

KW - Nitrogen analysis

KW - Nitrogen-to-protein conversion factor

KW - Protein

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DO - 10.1007/7651_2018_126

M3 - Chapter

C2 - 29675783

AN - SCOPUS:85076427397

T3 - Methods in Molecular Biology

SP - 233

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BT - Biofuels from Algae: Methods and Protocols

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Laurens L , Olstad-Thompson J, Templeton D. Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. In Spilling K, editor, Biofuels from Algae: Methods and Protocols: Methods in Molecular Biology. Humana Press Inc. 2020. p. 233-242. (Methods in Molecular Biology). doi: 10.1007/7651_2018_126, 10.1007/7651_2018_126

Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

Abstract

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Keywords

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