Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase: Article No. 4389

Roman Brunecky, Bryon Donohoe, Hui Wei, Ashutosh Mittal, Michael Himmel, Vladimir Lunin, Todd Shollenberger

Research output: Contribution to journalArticlepeer-review

9 Scopus Citations

Abstract

In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose ..beta..-1-4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.
Original languageAmerican English
Number of pages9
JournalScientific Reports
Volume7
DOIs
StatePublished - 2017

NREL Publication Number

  • NREL/JA-2700-67215

Keywords

  • AcCel5A endoglucanase
  • biomass
  • in planta expression
  • plant cell wall
  • recalcitrance

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