TY - JOUR
T1 - Towards the Integration of Dark- and Photo-Fermentative Waste Treatment. 2. Optimization of Starch-Dependent Fermentative Hydrogen Production
AU - Belokopytov, Boris F.
AU - Laurinavichius, Kestutis S.
AU - Laurinavichene, Tatyana V.
AU - Ghirardi, Maria L.
AU - Seibert, Michael
AU - Tsygankov, Anatoly A.
PY - 2009
Y1 - 2009
N2 - Eight natural microbial consortia collected from different sites were tested for dark, hydrogen production during starch degradation. The most active consortium was from silo pit liquid under mesophilic (37 °C) conditions. The fermentation medium for this consortium was optimized (Fe, NH4+, phosphates, peptone, and starch content) for both dark fermentation and for subsequent purple photosynthetic bacterial H2 photoproduction [Laurinavichene TV, Tekucheva DN, Laurinavichius KS, Ghirardi ML, Seibert M, Tsygankov AA. Towards the integration of dark and photo fermentative waste treatment. 1. Hydrogen photoproduction by purple bacterium Rhodobacter capsulatus using potential products of starch fermentation. Int J Hydrogen Energy 2008;33(23):7020-26], in the presence of the spent dark, fermentation effluent. The addition of Zn (10 mg L-1), as a methanogenesis inhibitor that does not inhibit purple bacteria at this concentration, also did not inhibit dark, fermentative H2 production. The influence of various fermentation end products at different concentrations (up to 30 g L-1) on dark, H2 production was also examined. Added lactate stimulated, but added isobutyrate and butanol strongly inhibited gas production. Under optimal conditions the fermentation of starch (30 g L-1) resulted in 5.7 L H2 L-1 of culture (1.6 mol H2 per mole of hexose) with the co-production mainly of butyrate and acetate.
AB - Eight natural microbial consortia collected from different sites were tested for dark, hydrogen production during starch degradation. The most active consortium was from silo pit liquid under mesophilic (37 °C) conditions. The fermentation medium for this consortium was optimized (Fe, NH4+, phosphates, peptone, and starch content) for both dark fermentation and for subsequent purple photosynthetic bacterial H2 photoproduction [Laurinavichene TV, Tekucheva DN, Laurinavichius KS, Ghirardi ML, Seibert M, Tsygankov AA. Towards the integration of dark and photo fermentative waste treatment. 1. Hydrogen photoproduction by purple bacterium Rhodobacter capsulatus using potential products of starch fermentation. Int J Hydrogen Energy 2008;33(23):7020-26], in the presence of the spent dark, fermentation effluent. The addition of Zn (10 mg L-1), as a methanogenesis inhibitor that does not inhibit purple bacteria at this concentration, also did not inhibit dark, fermentative H2 production. The influence of various fermentation end products at different concentrations (up to 30 g L-1) on dark, H2 production was also examined. Added lactate stimulated, but added isobutyrate and butanol strongly inhibited gas production. Under optimal conditions the fermentation of starch (30 g L-1) resulted in 5.7 L H2 L-1 of culture (1.6 mol H2 per mole of hexose) with the co-production mainly of butyrate and acetate.
KW - Dark fermentation
KW - Hydrogen production
KW - Starch degradation
KW - Waste treatment
UR - http://www.scopus.com/inward/record.url?scp=64549157146&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2009.02.042
DO - 10.1016/j.ijhydene.2009.02.042
M3 - Article
AN - SCOPUS:64549157146
SN - 0360-3199
VL - 34
SP - 3324
EP - 3332
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 8
ER -