Towards the Integration of Dark- and Photo-Fermentative Waste Treatment. 4. Repeated Batch Sequential Dark- and Photofermentation using Starch as Substrate

Tatyana V. Laurinavichene, Boris F. Belokopytov, Kestutis S. Laurinavichius, Anna N. Khusnutdinova, Michael Seibert, Anatoly A. Tsygankov

Research output: Contribution to journalArticlepeer-review

38 Scopus Citations

Abstract

In this study we demonstrated the technical feasibility of a prolonged, sequential two-stage integrated process under a repeated batch mode of starch fermentation. In this durable scheme, the photobioreactor with purple bacteria in the second stage was fed directly with dark culture from the first stage without centrifugation, filtration, or sterilization (not demonstrated previously). After preliminary optimization, both the dark- and the photo-stages were performed under repeated batch modes with different process parameters. Continuous H 2 production in this system was observed at a H 2 yield of up to 1.4 and 3.9 mole mole -1 hexose during the dark- and photo-stage, respectively (for a total of 5.3 mole mole -1 hexose), and rates of 0.9 and 0.5 L L -1 d -1, respectively. Prolonged repeated batch H 2 production was maintained for up to 90 days in each stage and was rather stable under non-aseptic conditions. Potential for improvements in these results are discussed.

Original languageAmerican English
Pages (from-to)8800-8810
Number of pages11
JournalInternational Journal of Hydrogen Energy
Volume37
Issue number10
DOIs
StatePublished - May 2012

NREL Publication Number

  • NREL/JA-2A00-55922

Keywords

  • Dark fermentation
  • Hydrogen production
  • Integrated system
  • Purple photosynthetic bacteria
  • Repeated batch cultivation

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