Tracking Dynamics of Plant Biomass Composting by Changes in Substrate Structure, Microbial Community, and Enzyme Activity

Hui Wei, Melvin P. Tucker, John O. Baker, Michelle Harris, Yonghua Luo, Qi Xu, Michael E. Himmel, Shi You Ding

Research output: Contribution to journalArticlepeer-review

35 Scopus Citations

Abstract

Background: Understanding the dynamics of the microbial communities that, along with their secreted enzymes, are involved in the natural process of biomass composting may hold the key to breaking the major bottleneck in biomass-to-biofuels conversion technology, which is the still-costly deconstruction of polymeric biomass carbohydrates to fermentable sugars. However, the complexity of both the structure of plant biomass and its counterpart microbial degradation communities makes it difficult to investigate the composting process. Results: In this study, a composter was set up with a mix of yellow poplar (Liriodendron tulipifera) wood-chips and mown lawn grass clippings (85:15 in dry-weight) and used as a model system. The microbial rDNA abundance data obtained from analyzing weekly-withdrawn composted samples suggested population-shifts from bacteria-dominated to fungus-dominated communities. Further analyses by an array of optical microscopic, transcriptional and enzyme-activity techniques yielded correlated results, suggesting that such population shifts occurred along with early removal of hemicellulose followed by attack on the consequently uncovered cellulose as the composting progressed. Conclusion: The observed shifts in dominance by representative microbial groups, along with the observed different patterns in the gene expression and enzymatic activities between cellulases, hemicellulases, and ligninases during the composting process, provide new perspectives for biomass-derived biotechnology such as consolidated bioprocessing (CBP) and solid-state fermentation for the production of cellulolytic enzymes and biofuels.

Original languageAmerican English
Article number20
Number of pages20
JournalBiotechnology for Biofuels
Volume5
Issue number4
DOIs
StatePublished - 2012

NREL Publication Number

  • NREL/JA-2700-56077

Keywords

  • Biofuels
  • Cellulase
  • Compost
  • Consolidated bioprocessing (CBP)
  • Enzymatic activity
  • Gene expression
  • Hemicellulose
  • Ligninase
  • Microbial community
  • Microbial rDNA abundance
  • Plant biomass
  • Solid-state fermentation
  • Yellow poplar

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