Two-Stage Dilute Acid-Organic Solvent Lignocellulosic Pretreatment for Enhanced Bioprocessing

Jonathan Stickel, Gary Brodeur, John Telotte, Subramanian Ramakrishnan

Research output: Contribution to journalArticlepeer-review

22 Scopus Citations


A two stage pretreatment approach for biomass is developed in the current work in which dilute acid (DA) pretreatment is followed by a solvent based pretreatment (N-methyl morpholine N oxide -- NMMO). When the combined pretreatment (DAWNT) is applied to sugarcane bagasse and corn stover, the rates of hydrolysis and overall yields (>90%) are seen to dramatically improve and under certain conditions 48 h can be taken off the time of hydrolysis with the additional NMMO step to reach similar conversions. DAWNT shows a 2-fold increase in characteristic rates and also fractionates different components of biomass -- DA treatment removes the hemicellulose while the remaining cellulose is broken down by enzymatic hydrolysis after NMMO treatment to simple sugars. The remaining residual solid is high purity lignin. Future work will focus on developing a full scale economic analysis of DAWNT for use in biomass fractionation.
Original languageAmerican English
Pages (from-to)621-628
Number of pages8
JournalBioresource Technology
StatePublished - 2016

NREL Publication Number

  • NREL/JA-5100-66759


  • biomass to biofuels
  • cellulose
  • enzymatic hydrolysis
  • multi stage treatment


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