Variation of S/G Ratio and Lignin Content in a Populus Family Influences the Release of Xylose by Dilute Acid Hydrolysis

Brian H. Davison, Sadie R. Drescher, Gerald A. Tuskan, Mark F. Davis, Nhuan P. Nghiem

Research output: Contribution to journalArticlepeer-review

155 Scopus Citations

Abstract

Wood samples from a second generation Populus cross were shown to have different lignin contents and S/G ratios (S: syringyl-like lignin structures; G: guaiacyl-like lignin structures). The lignin contents varied from 22.7% to 25.8% and the S/G ratio from 1.8 to 2.3. Selected samples spanning these ranges were hydrolyzed with dilute (1%) sulfuric acid to release fermentable sugars. The conditions were chosen for partial hydrolysis of the hemicellulosic fraction to maximize the expression of variation among samples. The results indicated that both lignin contents and S/G ratio significantly affected the yield of xylose. For example, the xylose yield of the 25.8% lignin and 2.3 S/G (high lignin, high S/G) sample produced 30% of the theoretical yield, whereas the xylose yield of the 22.7% lignin and 1.8 S/G (low lignin, low S/G) was 55% of the theoretical value. These results indicate that lignin content and composition among genetic variants within a single species can influence the hydrolyzability of the biomass.

Original languageAmerican English
Pages (from-to)427-435
Number of pages9
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume130
Issue number1-3
DOIs
StatePublished - 2006

NREL Publication Number

  • NREL/JA-510-40336

Keywords

  • Cell wall chemistry
  • Genetic variation
  • Hybrid poplar
  • Hydrolysis
  • Lignin

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