Visualization of Trichoderma reesei Cellobiohydrolase I and Endoglucanase I on Aspen Cellulose by Using Monoclonal Antibody-Colloidal Gold Conjugates

R. A. Nieves; R. P. Ellis; R. J. Todd; T. J.A. Johnson; K. Grohmann; M. E. Himmel

doi:10.1128/aem.57.11.3163-3170.1991

Visualization of Trichoderma reesei Cellobiohydrolase I and Endoglucanase I on Aspen Cellulose by Using Monoclonal Antibody-Colloidal Gold Conjugates

R. A. Nieves, R. P. Ellis, R. J. Todd, T. J.A. Johnson, K. Grohmann, M. E. Himmel

BioEconomy and Sustainable Transportation

National Renewable Energy Laboratory

Research output: Contribution to journal › Article › peer-review

31 Scopus Citations

Abstract

Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immobilization of cellulose microfibrils on copper electron microscopy grids, incubation of the cellulose-coated grids with cellulase(s), binding of MAb-colloidal gold conjugates to cellulase(s), and visualization via transmission electron microscopy. CBH I was seen bound to apparent crystalline cellulose as well as apparent amorphous cellulose. EG I was seen bound extensively to apparent amorphous cellulose with minimal binding to crystalline cellulose.

Original language	American English
Pages (from-to)	3163-3170
Number of pages	8
Journal	Applied and Environmental Microbiology
Volume	57
Issue number	11
DOIs	https://doi.org/10.1128/aem.57.11.3163-3170.1991
State	Published - 1991

NREL Publication Number

ACNR/JA-232-12726

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10.1128/aem.57.11.3163-3170.1991

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title = "Visualization of Trichoderma reesei Cellobiohydrolase I and Endoglucanase I on Aspen Cellulose by Using Monoclonal Antibody-Colloidal Gold Conjugates",

abstract = "Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immobilization of cellulose microfibrils on copper electron microscopy grids, incubation of the cellulose-coated grids with cellulase(s), binding of MAb-colloidal gold conjugates to cellulase(s), and visualization via transmission electron microscopy. CBH I was seen bound to apparent crystalline cellulose as well as apparent amorphous cellulose. EG I was seen bound extensively to apparent amorphous cellulose with minimal binding to crystalline cellulose.",

author = "Nieves, {R. A.} and Ellis, {R. P.} and Todd, {R. J.} and Johnson, {T. J.A.} and K. Grohmann and Himmel, {M. E.}",

year = "1991",

doi = "10.1128/aem.57.11.3163-3170.1991",

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volume = "57",

pages = "3163--3170",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Visualization of Trichoderma reesei Cellobiohydrolase I and Endoglucanase I on Aspen Cellulose by Using Monoclonal Antibody-Colloidal Gold Conjugates

AU - Nieves, R. A.

AU - Ellis, R. P.

AU - Todd, R. J.

AU - Johnson, T. J.A.

AU - Grohmann, K.

AU - Himmel, M. E.

PY - 1991

Y1 - 1991

N2 - Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immobilization of cellulose microfibrils on copper electron microscopy grids, incubation of the cellulose-coated grids with cellulase(s), binding of MAb-colloidal gold conjugates to cellulase(s), and visualization via transmission electron microscopy. CBH I was seen bound to apparent crystalline cellulose as well as apparent amorphous cellulose. EG I was seen bound extensively to apparent amorphous cellulose with minimal binding to crystalline cellulose.

AB - Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immobilization of cellulose microfibrils on copper electron microscopy grids, incubation of the cellulose-coated grids with cellulase(s), binding of MAb-colloidal gold conjugates to cellulase(s), and visualization via transmission electron microscopy. CBH I was seen bound to apparent crystalline cellulose as well as apparent amorphous cellulose. EG I was seen bound extensively to apparent amorphous cellulose with minimal binding to crystalline cellulose.

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U2 - 10.1128/aem.57.11.3163-3170.1991

DO - 10.1128/aem.57.11.3163-3170.1991

M3 - Article

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SN - 0099-2240

VL - 57

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ER -

Visualization of Trichoderma reesei Cellobiohydrolase I and Endoglucanase I on Aspen Cellulose by Using Monoclonal Antibody-Colloidal Gold Conjugates

Abstract

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