Yeast Adaptation on Softwood Prehydrolysate

    Research output: Contribution to journalArticlepeer-review


    Several strains and genera of yeast, including Saccharomyces cerevisiae D5A, Pachysolen tannophilus, S. cerevisiae K-1, Brettanomyces custersii, Candida shehatae, and Candida acidothermophilum, are screened for growth on dilute acid-pretreated softwood prehydrolysate. Selected softwood species found in forest underbrush of the western United States, which contain predominantly hexosanhemicellulose, were studied. This phase of the work emphasized debarked Douglas fir. The two best initial isolates were gradually selected for improved growth by adaptation to increasing prehydrolysate concentrations in batch culture, with due consideration of nutrient requirements. Microaerophilic conditions were evaluated to encourage tolerance of pretreatment hydrolysate, as well as ethanolproduct. Adaptation and simultaneous saccharification and fermentation (SSF) results are used to illustrate improved performance with an adapted strain, compared to the wild type.
    Original languageAmerican English
    Pages (from-to)137-148
    Number of pages12
    JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
    StatePublished - 1998

    NREL Publication Number

    • NREL/JA-580-22883


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